基于GC-MS代谢组学技术对内脂素调控雏鸡采食机制的研究

doi:10.11843/j.issn.0366-6964.2017.07.009

Abstract

Abstract:

The objectives of the study were to explore the molecular mechanism of Visfatin regulating feed intake, and to further improve the theory of appetite regulation and energy balance in poultry.In this study, 20 healthy Roman Brown chicks at the age of 1 day old were randomly divided into 4 groups(group C, L, M, H). When the chicks grew up to the age of 10 days old fed with conventional commercial diet, they were intracerebroventricularly injected with various concentrations of human recombinant Visfatin(0, 40, 200, 400 ng) in hypothalamus, respectively. The cumulative feed intake was recorded after injection for 60 min. The chicks were then euthanized and the hypothalamus were collected. Metabolomics analysis was performed through GC-MS system. The PLS-DA model was built and the metabolic pathways of differential metabolites were constructed by KEGG. The results showed that the cumulative feed intakes of chicks in M and H groups were increased significantly compared to that in C group (P <0.05). A total of 73 differential metabolites, which were enriched in 51 pathways, were identified by GC-MS analysis.The differential metabolites in hypothalamus were closely related to carbohydrate, lipid and amino acid metabolism, and fatty acid biosynthesis pathway was one of the most significantly enriched pathways. The main reason for Visfatin promoting food intake is the change of steady state due to decrease of central nutrients.

CLC Number:

S831.2

LIU Xue-lian, ZHANG Pan-pan, TIAN Fang-yuan, LI Zhun, LI Zhuan-jian, HAN Rui-li, SUN Gui-rong, JIANG Rui-rui, LIU Xiao-jun, KANG Xiang-tao, TIAN Ya-dong. Research of Visfatin Regulating Chicks Feed Intake Based on GC-MS Metabolomics[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1251-1259.

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Research of Visfatin Regulating Chicks Feed Intake Based on GC-MS Metabolomics

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